Spread of Botrytis cinerea Strains with Multiple Fungicide Resistance in German Horticulture

Spread of Botrytis cinerea Strains with Multiple Fungicide Resistance in German Horticulture

Botrytis cinerea is a major plant pathogen, causing gray mold rot in a variety of cultures. Repeated fungicide applications are common but have resulted in the development of fungal populations with resistance to one or more fungicides. In this study, we have monitored fungicide resistance frequencies and the occurrence of multiple resistance in Botrytis isolates from raspberries, strawberries,...

Detection of Botrytis cinerea field isolates with multiple fungicide resistance

Multiple Fungicide Resistance in Botrytis: A Growing Problem in German Soft-Fruit Production

In the mild and humid climate of northwestern Europe, fungal pre-harvest rots are a major factor limiting the production of soft-fruits such as strawberries and raspberries. By far the most important fungal disease is grey mould caused by Botrytis cinerea Pers.:Fr., now known to be an aggregate of at least two distinct species (Fournier et al., 2005; Giraud et al., 1999). Primary infections are...

Suspension Array for Multiplex Detection of Eight Fungicide-Resistance Related Alleles in Botrytis cinerea

A simple and high-throughput assay to detect fungicide resistance is required for large-scale monitoring of the emergence of resistant strains of Botrytis cinerea. Using suspension array technology performed on a Bio-Plex 200 System, we developed a single-tube allele-specific primer extension assay that can simultaneously detect eight alleles in one reaction. These eight alleles include E198 an...

Comparative Genomics of Botrytis cinerea Strains with Differential Multi-Drug Resistance

1 Laboratory of Plant Pathology, Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Volos, Greece, Department of Electrical and Computer Engineering, Aristotle University of Thessaloniki, Thessaloniki, Greece, Department of Ichthyology and Aquatic Environment, University of Thessaly, Volos, Greece, Centre for Research and Technology Hellas, Institute of Ap...